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Confocal immunofluorescence analysis of HLB foci in cells synchronized at the early S-phase. A, double staining for dmGapdh (red) and HLB (green) is shown. Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue). B, the image represents the z-stack projection of 17 confocal sections (stack z-spacing, 0.44 μm), showing both XZ and YZ sections. Nuclear dmGapdh was co-localized with HLB (arrowheads). Scale bar, 5 μm.
