TABLE 3.
Voltage dependence and inactivation kinetics parameters for carboxyl terminal mutants
Values presented are means ± S.D.
| Channela | Voltage dependence |
Kinetics of inactivation |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Inactivation |
Activation |
||||||||||
| V½ | a | n | V½ | z | n | τSlow | ASlow | τFast | AFast | n | |
| mV | mV | mV | e0 | ms | % | ms | % | ||||
| Nav1.3 | −16.2 ± 0.8 | 4.7 ± 0.5 | 8 | −14.6 ± 2.4 | 6.9 ± 0.6 | 8 | 28.4 ± 4.0 | 100 | NAb | NA | 14 |
| K1826E | −27.5 ± 1.6c | 8.3 ± 1.0c | 14 | −14.0 ± 1.0 | 6.2 ± 0.2 | 5 | 42.9 ± 5.8 | 38 ± 4 | 9.8 ± 1.4 | 62 ± 4 | 5 |
| K1826D | −28.6 ± 1.6c | 8.6 ± 0.5c | 10 | −15.4 ± 0.7 | 6.9 ± 0.6 | 9 | 50.8 ± 10.6 | 34 ± 9 | 11.3 ± 1.5 | 66 ± 9 | 9 |
| K1826R | −18.3 ± 1.2 | 4.8 ± 0.9 | 12 | −14.0 ± 1.4 | 5.7 ± 0.8 | 6 | 25.5 ± 3.2 | 100 | NA | NA | 6 |
| K1826A | −25.3 ± 1.3c | 7.1 ± 0.4c | 11 | −12.5 ± 2.2 | 5.4 ± 0.4 | 8 | 38.5 ± 7.2 | 31 ± 6 | 12.0 ± 2.3 | 69 ± 6 | 6 |
| D1827E | −18.2 ± 2.1 | 4.6 ± 0.7 | 8 | −12.3 ± 0.4 | 5.6 ± 0.1 | 5 | 41.0 ± 1.7 | 100 | NA | NA | 5 |
| A1856I | −21.1 ± 1.6c | 5.6 ± 0.9 | 7 | −21.0 ± 1.8c | 8.0 ± 1.1 | 7 | 24.7 ± 3.8 | 100 | NA | NA | 7 |
| A1856I/I1857V | −18.2 ± 0.9 | 4.5 ± 0.5 | 17 | −16.4 ± 1.5 | 8.8 ± 1.6 | 12 | 22.4 ± 1.8 | 100 | NA | NA | 8 |
| N1861A | −17.4 ± 0.7 | 4.8 ± 0.6 | 7 | −13.4 ± 2.7 | 6.2 ± 0.9 | 6 | 29.8 ± 5.9 | 100 | NA | NA | 5 |
| A1856I/I1857V/N1861A | −17.6 ± 1.2 | 3.9 ± 0.4 | 13 | −15.7 ± 2.9 | 8.3 ± 1.8 | 5 | 24.4 ± 3.5 | 100 | NA | NA | 7 |
| C1864R | −18.4 ± 1.3 | 4.6 ± 0.5 | 17 | −15.5 ± 2.0 | 6.8 ± 0.7 | 18 | 25.4 ± 2.5 | 100 | NA | NA | 8 |
| C1864R/R1870K | −16.6 ± 1.4 | 4.6 ± 0.4 | 18 | −14.4 ± 0.6 | 6.9 ± 0.7 | 9 | 26.9 ± 1.5 | 100 | NA | NA | 6 |
| C1864R/R1870K/L1871V | −18.0 ± 0.9 | 4.7 ± 0.4 | 11 | −14.3 ± 1.2 | 6.2 ± 0.4 | 8 | 24.6 ± 2.1 | 100 | NA | NA | 6 |
| CRL-N1873K | −19.1 ± 1.7 | 4.2 ± 0.2 | 9 | −20.0 ± 1.3c | 6.8 ± 2.0 | 9 | 21.4 ± 1.3 | 100 | NA | NA | 5 |
| CRLN-I1874V | −17.2 ± 0.9 | 4.2 ± 0.4 | 6 | −14.5 ± 2.0 | 7.2 ± 0.8 | 6 | 25.2 ± 2.8 | 100 | NA | NA | 5 |
| CRLNI-K1877I | −17.0 ± 1.4 | 4.0 ± 0.2 | 6 | −18.3 ± 0.8 | 7.1 ± 0.8 | 5 | 26.8 ± 2.5 | 100 | NA | NA | 5 |
| CRLNIK-D1879K | −18.2 ± 0.8 | 4.8 ± 0.4 | 6 | −14.2 ± 0.9 | 6.5 ± 0.2 | 8 | 29.6 ± 3.2 | 100 | NA | NA | 8 |
| CRLNIKD-E1881D | −17.8 ± 0.9 | 4.2 ± 0.2 | 11 | −15.6 ± 0.8 | 7.9 ± 0.9 | 8 | 22.4 ± 2.8 | 100 | NA | NA | 7 |
| CRLNIKDE-T1882K | −20.2 ± 1.1c | 4.2 ± 0.2 | 13 | −15.6 ± 1.4 | 7.4 ± 0.9 | 12 | 21.2 ± 2.2 | 100 | NA | NA | 7 |
| K1826E/D1827E | −25.3 ± 0.7c | 8.1 ± 0.4c | 5 | −16.4 ± 2.7 | 10.0 ± 1.8 | 5 | 43.2 ± 3.9 | 25 ± 2 | 8.0 ± 0.4 | 75 ± 2 | 5 |
| D1827E/A1856I/I1857V | −18.2 ± 1.3 | 5.0 ± 0.6 | 6 | −13.8 ± 1.3 | 6.2 ± 0.6 | 5 | 39.1 ± 10.6 | 100 | NA | NA | 5 |
| K1826E/D1827E/A1856I/I1857V | −28.2 ± 0.9c | 8.5 ± 0.8c | 8 | −14.2 ± 2.3 | 6.0 ± 0.6 | 5 | 32.2 ± 2.5 | 35 ± 11 | 6.7 ± 1.3 | 64 ± 11 | 5 |
| Nav1.2 | −36.0 ± 3.2c | 8.0 ± 0.6c | 8 | −13.4 ± 1.6 | 5.2 ± 0.4 | 6 | 17.6 ± 3.0 | 38 ± 4 | 4.2 ± 0.9 | 62 ± 4 | 8 |
| E1880K | −22.8 ± 2.8d | 6.5 ± 0.7d | 13 | −17.4 ± 1.4 | 5.2 ± 0.2 | 9 | 22.7 ± 3.9 | 100 | NA | NA | 9 |
| E1881D | −31.8 ± 1.8d | 7.1 ± 0.4d | 9 | −14.6 ± 0.8 | 5.7 ± 0.3 | 5 | 27.2 ± 5.1 | 38 ± 4 | 7.4 ± 0.4 | 62 ± 4 | 5 |
| E1880K/E1881D | −20.8 ± 1.7d | 5.2 ± 0.3d | 5 | −16.0 ± 1.3 | 5.9 ± 0.2 | 5 | 22.3 ± 3.6 | 100 | NA | NA | 5 |
a Mutant channels are indicated by the amino acid substitution and by the initial amino acid in subsequent lines (e.g. C1864R/R1870K/L1871V is CRL).
b NA, not applicable because current trace was efficiently fitted with a single exponential equation.
c Significantly different from Nav1.3, p < 0.001.
d Significantly different from Nav1.2, p < 0.001.