FIGURE 4.

Resveratrol lowers Aβ levels by activating AMPK in primary neurons. A, shown is pAMPK (panels a, d, and g), NeuN (panels b and e), GFAP (panel h), and merged (panels c, f, and i) staining of sagittal brain sections of an adult mouse. Hippocampus (panels a–c and g–i) and cerebral cortex (panels d–f) are shown. B, shown is phase contrast (PC), 4′,6-diamidino-2-phenylindole (DAPI), NeuN, pAMPK, and merged staining of 15 days in vitro primary neuronal cultures from J20 APP transgenic mice. C–H, WB analyses of pAMPK, AMPK, pACC, ACC, APP, and actin (C, E, and G) and ELISA measurements of secreted Aβ1-x levels (D, F, and H) in 15 days in vitro J20 mouse primary neurons treated for 24 h with the indicated concentrations of resveratrol (RSV, C and D) or AICAR (E and F). Primary neurons in G and H were treated for 24 h with 80 μm resveratrol or its vehicle (DMSO, control (CTRL)) in the absence or presence of compound C (CC, 20 μm). **, p < 0.01; ns, not significant (Student's t test).