FIGURE 4.
Panx1-GFP is highly mobile at all plasma membrane locations. Panx1-GFP was localized to three distinct plasma membrane domains of BICR-M1Rk cells, as depicted by the schematic diagram (A). Fluorescent images of Panx1-GFP were superimposed with DIC images to highlight the microenvironment surrounding the cell being analyzed (A–D). Selected cell regions where Panx1-GFP was localized at the three distinct plasma membrane domains were photobleached, and fluorescence recovery back into the photobleached areas was assessed and normalized over 60 s (B–D). Panx1-GFP recovery within the photobleached area was not significantly different (p > 0.05) among all three domains (E). Bars, 10 μm. n = 6–9 per plasma membrane domain collected from three independent experiments.