FIGURE 6.
Panx3-GFP is highly mobile at all plasma membrane domains. Panx3-GFP was localized to three distinct plasma membrane domains of BICR-M1Rk cells co-expressing Panx3 (A–C). Selected cell surface regions containing Panx3-GFP were photobleached, and fluorescence recovery back into the photobleached areas was assessed and normalized over the time course of 60 s. The percentage of Panx3-GFP recoverable fraction was not found to be significantly different among all three plasma membrane domains examined (p > 0.05) (D). Bars, 10 μm. n = 12–25 per plasma membrane domain collected from three independent experiments.