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. 2010 Jan 20;285(12):9190–9201. doi: 10.1074/jbc.M109.051938

FIGURE 1.

FIGURE 1.

ABA sensitivity of transgenic Arabidopsis overexpressing RPK1. A, expression levels of RPK1 in transgenic plants. Total RNAs were extracted from transgenic plants carrying the 35S vector (vector control (VC)) and from four transgenic lines of 35S:RPK1 (L1–L4; Columbia ecotype) and were blotted and hybridized with a cDNA probe for RPK1. Protein blot analysis was performed with microsomal fractions of 35S:RPK1 lines (L1–L4) using an anti-RPK1 antibody (23). B, 35S:RPK1 (lines L1–L3) and vector control plants grown on GM-agar plates for 2 weeks. C and D, assay for root growth inhibition by ABA. Young seedlings of 35S:RPK1 (lines L1 and L2), vector control, wild type Arabidopsis (WS), and rpk1-1 were transferred to ABA-containing medium and incubated for 10 days. The relative root lengths were measured and are shown as a percentage of the root lengths grown in the absence of ABA. Values are the means ± S.D. (n = 50). The asterisks indicate statistically significant differences in comparisons between WS and rpk1-1, determined by Student's t test, or in comparisons between vector control and 35S:RPK1, evaluated by one-way ANOVA with Tukey's HSD (*, p < 0.0001; **, p < 0.005). Bars, 1 cm.