TABLE 2.

Binding parameters of full-length pET28a-Mp1p with fatty acids and pET28a-LBD2 (WT or mutant) with palmitoleic acid

All of the binding parameters were derived from ITC assays at 25 °C as described under “Experimental Procedures.” K_d is the dissociation constant, and n is the number of binding sites.

Protein	Fatty acid	Kd	ΔH	n
		μm	kJ/mol
Mp1p	Palmitoleic acid	6.34	−43.77	2.16
Mp1p	Oleic acid	4.15	−39.38	2.21
Mp1p	Arachidonic acid	2.36	−43.99	2.31
LBD2	Palmitoleic acid	2.34	−51.58	1.00
LBD2 S313A	Palmitoleic acid	1.24	−58.48	0.69
LBD2 S313A/S332A	Palmitoleic acid	18.82	−44.50	0.76