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. 2010 Jan 15;285(12):9221–9232. doi: 10.1074/jbc.M109.073650

FIGURE 1.

FIGURE 1.

DAX-1 represses the transcriptional activity of LXRα. A, Western blot analysis. Western blot analysis was performed using the protein extracts from mouse tissues (upper panel) and cell lines (lower panel). B and C, HepG2 (B) and 293T (C) cells were transfected with pcDNA3-HA-LXRα (200 ng), HA-DAX-1(50, 100, and 200 ng), and LXRE-luc (200 ng). As positive control, we transfected HA-SHP and HA-LXRα and LXRE-luc in the presence of ligand. Effect of DAX-1 alone with basal reporter activity was also shown. D, 293T cells were transfected with HA-LXRα (10 μg) and FLAG-hDAX-1 (5 and 10 μg). E, HepG2 cells were transfected with sihDAX-1-1 (200 pmol) or sihDAX-1-2 (50 and 200 pmol), and 24 h later HA-LXRα (200 ng) and LXRE-Luc (200 ng) were transfected. After 24 h, the cells were harvested, and luciferase and β-galactosidase assays were performed. F, HepG2 cells were transfected with sihDAX-1-1 (200 pmol) and sihDAX-1-2 (50, 100, and 200 pmol). After 48 h transfection cells (D and E) were harvested for Western blot analysis with the indicated antibodies.