FIGURE 6.

DAX-1 lowers serum triglyceride and lipid accumulation in liver. Comparison of DAX-1 and lipogenic gene expressions in normal and db/db mice. A, quantitative PCR analysis of hepatic mRNA levels of DAX-1, SRC-1, PGC-1a, SREBP-1C, FAS and acetyl-coenzyme A carboxylase in normal and db/db mice (*, p < 0.01; n = 4). B, DAX-1 decreases T7-mediated lipogenic gene expression in mice. Male 8-week-old C57BL6 mice were provided with the meal form of a standard rodent diet. T0901317 (LXR agonist, 50 mg/kg body weight) or vehicle (1% methylcellulose and 1% Tween 80) were administered by oral gavage each day for 1 week. Recombinant adenovirus (0.5 × 10⁹ plaque-forming unit) GFP (n = 5) or DAX-1 (n = 5) were delivered by tail vein injection on the 4th day of oral gavage. Three days after adenovirus GFP (n = 3) or DAX-1 (n = 3) injection, mice were sacrificed, and the expressions of SREBP-1c, FAS, and ACC1α were analyzed by real time quantitative RT-PCR. All data were normalized to ribosomal L32 expression. C, liver triglyceride level is decreased by DAX-1. Hepatic TAG levels were analyzed from mouse liver tissue infected with adenoviruses as in B. D, DAX-1 decreases the lipid accumulation in liver. Oil Red O staining was performed from the liver samples as in B. Data in A–C are represented as mean ± S.D.