FIGURE 3.

Characterization of astroglial α-synuclein inclusions. A–D, inclusion bodies in co-cultured primary astrocytes were stained with thioflavin S (A) or with antibodies for ubiquitin (B), Hsp70/Hsc70 (C), and 20 S proteasome α-subunit (D). Inclusion bodies that are positive for the markers are indicated with arrowheads, and the ones negative are marked with arrows. Scale bars, 20 μm. E, quantitative analysis of inclusion bodies for each marker. Experiments were repeated three times, and more than one hundred cells were counted per slide.