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. Author manuscript; available in PMC: 2011 Mar 1.

Published in final edited form as: Anal Chem. 2010 Mar 1;82(5):1942–1948. doi: 10.1021/ac9026862

Table 2.

Repeatability of LC-MS/MS Measurements of Serum 25(OH)D₃ and 25(OH)D₂

25(OH)D₃
Serum	Set	Mean ng/g	Within-set CV, %	Overall Mean, ng/g	Between-set CV, %
1	1	6.31	0.4	6.31	0.6
	2	6.27	0.2
	3	6.35	0.4
2	1	15.30	0.4	15.29	0.3
	2	15.28	0.5
	3	15.28	0.2
3	1	26.97	0.1	27.00	0.2
	2	27.01	0.3
	3	26.95	0.3

25(OH)D₂
1	1	0.86	0.9	0.86	2.0
	2	0.85	2.9
	3	0.86	2.0