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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Neurochem. 2009 Jul 8;110(6):1806–1817. doi: 10.1111/j.1471-4159.2009.06276.x

Figure 4.

Figure 4

Human AHCY 5′-FR reporter gene and EMS assays. (A) and (B) AHCY 5′-FR reporter gene constructs were used to transfect HEK293T and HepG2 cells. Luciferase activity is expressed as a percentage of the value for WT constructs. Each bar represents the average of 6 independent experiments (mean ± SD), * p<0.001 by ANOVA (C) Human AHCY 5′-FR C(-34)T SNP effect on nuclear protein binding. Nuclear extract from HEK293T and HepG2 cells was incubated with the WT probe containing C(-34), or with the variant probe containing T(-34).