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. 2010 Mar;12(3):235–243. doi: 10.1593/neo.91646

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Copyright © 2010 Neoplasia Press, Inc. All rights reserved

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To assess the status of catecholamine storing vesicles, immunohistochemistry was performed for chromogranin A in Rb1^F2/F2:Trp53^F2-10/F2-10 (A) and Rb1^F2/F2:Trp53^F2-10/F2-10:TEC1 (B) and synaptophysin in Rb1^F2/F2:Trp53^F2-10/F2-10 (C) and Rb1^F2/F2:Trp53^F2-10/F2-10:TEC1 (D) adrenal medullas. Scale bar, 50 µm. Electron micrographs of Rb1^F2/F2:Trp53^F/F (E; original magnification, x50,000) and Rb1^F2/F2:Trp53^F/F:TEC1 (F; original magnification, x50,000) chromaffin cells showing the overall density and morphology of vesicles (arrow). H&E staining of Rb1^F2/F2:Trp53^F2-10/F2-10 (G) and Rb1^F2/F2:Trp53^F2-10/F2-10:TEC1 (H) heart sections that shows structural remodeling of the Rb1^F2/F2:Trp53^F2-10/F2-10:TEC1 hearts (as indicated by arrows).