Figure 3.

Fzd 9 is necessary for the effects of iloprost reversal of anchorage independent growth. (A and B) A549 and H661 cells were grown in soft agar exposed to siRNA knockdown of Fzd 9 or the PPARγ antagonist compound T0070907 at a concentration of 5 µM in the absence or presence of 10 µM iloprost. The colonies were incubated for several weeks and subsequently counted as previously described in Materials and Methods. (C and D) The H157 and H1703 cell lines encoding stable empty vector LPCX/LNCX or Fzd 9 was grown in soft agar in the absence or presence of 10 µM iloprost. The colonies were incubated for several weeks and subsequently counted through Metamorph (Downingtown, PA).