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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cell Calcium. 2010 Jan 6;47(2):103–111. doi: 10.1016/j.ceca.2009.12.007

Figure 2.

Figure 2

Accumulation of storage bodies in cells treated with the intracellular Ca2+ chelator BAPTA-AM and with the H+ pump inhibitor Bafilomycin. HeLa cells were treated for 24 hours with 1 µM bafilomycin and 10 µM BAPTA. TRPML1 knockdown cells were analyzed shown 24 hours after transfection. Electron microscopic assays were performed as described before [19]. White arrows indicate dense storage bodies. Black arrows indicate large vacuolar structures that are present in BAPTA-treated but not in TRPML1 knockdown or Bafilomycin-treated cells.