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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Nat Chem Biol. 2009 Oct 25;5(12):913–919. doi: 10.1038/nchembio.242

Figure 2.

Figure 2

Curlicide inhibition of in vitro CsgA polymerization. (a) The fluorescence of freshly purified CsgA mixed with 25 μM ThT, and respective curlicides, was measured at 495 nm after excitation at 438 nm. Data points corresponding to 60-min intervals are displayed. (b) Reductions in ThT fluorescence corresponded to reduced CsgA polymerization. Soluble CsgA levels were confirmed by SDS-PAGE and western analysis using anti-CsgA antibodies. Polymerized CsgA is SDS insoluble, and migration is impeded during electrophoresis.

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