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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cell Signal. 2009 Nov 20;22(4):590–599. doi: 10.1016/j.cellsig.2009.11.011

Figure 2.

Figure 2

Figure 2

Figure 2

The AMPK activator AICAR enhances and the AMPK inhibitor Compound C (Comp C or CC) attenuates CLA-induced cleavage of PARP, caspase 9 and caspase 3. A and B, TM4t cells were treated with or without 40 μM of t10,c12-CLA and/or 500 μM AICAR (A) or 20 μM Compound C (B) for various times and cleavage of PARP, caspase-9 and caspase-3 was evaluated by western blot. C and D, TM4t cells were treated for 72 hr with or without 40 μM of t10,c12-CLA and/or various concentrations of Compound C, photographed under the 10× microscope objective (C), then viable cell number estimated by the SRB assay on the attached (viable) cell monolayer (D). Compound C alone at a concentration of 20 μM modestly decreased cell number (P<0.05, left panel). The data in the right panel of D show the ratio of the ODs from the SRB assay from CLA-treated to untreated cells. The asterisk denotes a statistically significant difference of the 20 μM compound C group from each of the other groups.