Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2011 Apr 1.

Published in final edited form as: Cell Signal. 2009 Nov 20;22(4):590–599. doi: 10.1016/j.cellsig.2009.11.011

Decreased cellular energy levels are not responsible for activation of AMPK. A. Intracellular ATP levels, measured using a luminescence assay, are maintained in cells treated with t10,c12-CLA. TM4t cells were treated without or with 40 μM t10,c12-CLA for 24, 48, or 72 hr and cells harvested for measurement of ATP levels. Each bar represents the mean ± SEM of triplicate wells from a representative experiment. *, indicates a statistically significant difference compared to the control at the same time point. B. Attenuation of LKB1 expression by siRNA does not affect CLA-induced activation of AMPK. TM4t cells were transfected with LKB1 siRNA or control siRNA for 6 hr followed by treatment without or with 40 μM t10,c12-CLA for 24, 48 or 72 hr. Cells were harvested, and proteins evaluated by western blot.

Decreased cellular energy levels are not responsible for activation of AMPK. A. Intracellular ATP levels, measured using a luminescence assay, are maintained in cells treated with t10,c12-CLA. TM4t cells were treated without or with 40 μM t10,c12-CLA for 24, 48, or 72 hr and cells harvested for measurement of ATP levels. Each bar represents the mean ± SEM of triplicate wells from a representative experiment. *, indicates a statistically significant difference compared to the control at the same time point. B. Attenuation of LKB1 expression by siRNA does not affect CLA-induced activation of AMPK. TM4t cells were transfected with LKB1 siRNA or control siRNA for 6 hr followed by treatment without or with 40 μM t10,c12-CLA for 24, 48 or 72 hr. Cells were harvested, and proteins evaluated by western blot.