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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: J Biol Chem. 2006 Dec 12;282(6):3713–3719. doi: 10.1074/jbc.M609228200

FIGURE 1. In vitro polymerization of CsgA measured by ThT fluorescence, CD, and TEM.

FIGURE 1

A, the fluorescence of freshly purified CsgA mixed with 25 μm ThT was measured in 10-min intervals at 495 nm after excitation at 438 nm. B, circular dichroism analysis of 10 μm CsgA immediately after purification, 2 days after purification, 7 days after purification, and 15 days after purification. CsgA was incubated at room temperature without shaking after purification. C–E, TEM micrographs of 30 μm CsgA after incubation at room temperature for the indicated times. Scale bar, 500 nm.