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. Author manuscript; available in PMC: 2010 Mar 15.

Published in final edited form as: Eur J Immunol. 2008 May;38(5):1194–1203. doi: 10.1002/eji.200737882

Synergistic IFN-β induction occurs in B27-Tg macrophages and correlates with increased XBP-1 splicing. BM-derived macrophages from F344 B27-Tg (ν) or WT control rats () were activated with IFN-γ for 20 h, then stimulated with 10 ng/ml LPS for 1–4 h. (A) Relative IFN-β transcripts were measured by qPCR. In (B), XBP-1 splicing in WT () and B27-Tg (ν) macrophages was determined as described in the legend to Figure 6. IFN-γ activated macrophages from Lewis WT (), B7-Tg(■), or B27-Tg (ν) rats, were stimulated with LPS for 2 h. IFN-β message levels were determined by qPCR (C) and XBP-1 splicing by PCR (D) as in (B). Results in A are the average of duplicates and are representative of 3 experiments. Results shown in B-D are mean ±SD and are representative of at least 2 experiments. In (B) p<0.05 for B27-Tg vs. WT, where it is barely detected. In (C and D) asterisks (*) indicate p<0.05 for B27-Tg vs. B7-Tg and WT. Representative XBP-1 splicing gel lanes in B and D are in the same sequence as the quantitative data.

Inline graphic — Synergistic IFN-β induction occurs in B27-Tg macrophages and correlates with increased XBP-1 splicing. BM-derived macrophages from F344 B27-Tg (ν) or WT control rats () were activated with IFN-γ for 20 h, then stimulated with 10 ng/ml LPS for 1–4 h. (A) Relative IFN-β transcripts were measured by qPCR. In (B), XBP-1 splicing in WT () and B27-Tg (ν) macrophages was determined as described in the legend to Figure 6. IFN-γ activated macrophages from Lewis WT (), B7-Tg(■), or B27-Tg (ν) rats, were stimulated with LPS for 2 h. IFN-β message levels were determined by qPCR (C) and XBP-1 splicing by PCR (D) as in (B). Results in A are the average of duplicates and are representative of 3 experiments. Results shown in B-D are mean ±SD and are representative of at least 2 experiments. In (B) p<0.05 for B27-Tg vs. WT, where it is barely detected. In (C and D) asterisks (*) indicate p<0.05 for B27-Tg vs. B7-Tg and WT. Representative XBP-1 splicing gel lanes in B and D are in the same sequence as the quantitative data.