. 1997 Dec 9;94(25):13786–13791. doi: 10.1073/pnas.94.25.13786

Table 1.

Titers of recombinants (pfu/ml) in SK-N-MC cells in the presence of 2 mM Gua-HCl

Combination	Experiment no.
Combination	I	II	III	IV	V	VI
Extract A
PV1(RIPO)g^r RNA in vitro virus	<20	<20	<20	<20
	(0)	(0)	(0)	(0)
PV1(M)g^s RNA in vitro virus	<20	<20	<20	<20
	(0)	(0)	(0)	(0)
PV1(M)g^s RNA + PV1(RIPO)g^r RNA coreplicated in vitro	60	120	80	60
PV1(M)g^s RNA + PV1(RIPO)g^r RNA coreplicated in vitro	(3)	(6)	(4)	(3)
PV1(M)g^s RNA in vitro virus + PV1(RIPO)g^r RNA in vitro virus	<20	<20	<20	<20
	(0)	(0)	(0)	(0)
PV1(M)g^s RNA + PV1(RIPO)g^r RNA	<20	<20	<20	<20
	(0)	(0)	(0)	(0)
Extract B
PV1(M)g^s RNA + PV1(RIPO)g^r RNA coreplicated in vitro	180	200	200	160	160	160
PV1(M)g^s RNA + PV1(RIPO)g^r RNA coreplicated in vitro	(9)	(10)	(10)	(8)	(8)	(8)
PV1(M)g^s RNA + PV1(RIPO)g^r RNA coreplicated in vitro with 2	<20	20	<20	<20	20	<20
mM Gua-HCl	(0)	(1)	(0)	(0)	(1)	(0)

Each 50-μl reaction mixture was spread on a single plate. The total number of plaques obtained (in parentheses) and the approximate titer (pfu/ml) are given for each experiment. The reactions in A and B were carried out using separate HeLa S10 extracts.