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. 1997 Dec 9;94(25):13786–13791. doi: 10.1073/pnas.94.25.13786

Table 3.

Yield of parental and recombinant viruses in the presence of Gua-HCl

50-μl reactions +Gua-HCl PV1(M)gs (SK-N-MC)
PV1(RIPO)gr (HeLa + Gua-HCl)
Recombinants (SK-N-MC + Gua-HCl)
F
D P T D P T D P T
I 2.0  ×  102 0 <200 2.0  ×  103 10 2.0  ×  104 29 1 29 1.5  ×  10−3
II 2.0  ×  102 0 <200 2.0  ×  103 8 1.6  ×  104 29 0 <29 <1.5  ×  10−3
III 2.0  ×  102 0 <200 2.0  ×  103 5 1.0  ×  104 29 0 <29 <1.5  ×  10−3
IV 2.0  ×  102 0 <200 2.0  ×  103 7 1.4  ×  104 29 0 <29 <1.5  ×  10−3
V 2.0  ×  102 0 <200 2.0  ×  103 6 1.2  ×  104 29 0 <29 <1.5  ×  10−3
VI 2.0  ×  102 0 <200 2.0  ×  103 4 0.8  ×  104 29 0 <29 <1.5  ×  10−3
VII 20 0 <20
VIII 20 0 <20
    Avg. <8 1.3  ×  104 ≲5 ≲3.6  ×  10−4

D, dilution factor; P, no. of plaques; T, titer in pfu/ml; F, frequency of recombination.