Table 2.

Characterization of the ATP-induced inward current in TRH-responsive rat pituitary cells

Treatment	Amplitude, pA	τd, s	τoff, s	EC50, μM	Recovery, %
Controls	15 ± 3.4	11 ± 2	0.31 ± 0.03	27 ± 5	33 ± 5
+IVM	64 ± 11*	28 ± 3*	20 ± 1.8*	7.4 ± 1.9*

Data are means ± SE from 45 (controls) and 26 (with ivermectin, +IVM) recordings in thyrotropin-releasing hormone (TRH)-responsive cells. IVM (3 μM) or control solution was applied for 2 min before agonist application. Cells were stimulated with 100 μM ATP to estimate maximum amplitude. Cells were stimulated with 1, 5, 10, 50, and 100 μM ATP to estimate EC₅₀ values. Recovery was considered the percentage of the amplitude observed during the secondary agonist application after a 3-min washout period. τ_d, Desensitization time constant; τ_off, deactivation time constant.

^*P < 0.05, control vs. IVM-treated cells.