Fig. 4.
Effects of 8-pCPT-2′-O-Me-cAMP-AM on intracellular Ca2+ concentration ([Ca2+]i) in a human islet. A1: ESCA-AM (1 μM; application indicated by the horizontal bar) stimulated an increase of [Ca2+]i in a human islet equilibrated in standard extracellular saline (SES) containing 5.6 mM glucose. A2: the increase of [Ca2+]i imaged in this same islet at selected time points. Yellow bracket indicates the region from which ratiometric measurements of fura-2 fluorescence were obtained. Calibration bar indicates 65 μm. Findings are representative of results obtained in 3 experiments using 5 islets from 3 donors.