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. 2009 Dec 8;298(3):E419–E428. doi: 10.1152/ajpendo.00417.2009

Fig. 3.

Fig. 3.

SIRT1 is the target for the resveratrol and SRT1720 anti-inflammatory effects. After pretreatment of 50 μM resveratrol (A) or 1 μM SRT1720 (B) for 1 h, RAW264.7 cells electroporated with control or SIRT1 siRNA were stimulated with or without 100 ng/ml LPS for 10 min and then lysed, and immunoblotting was performed with indicated antibodies. Scanned bar graphs show means ± SE, and values are expressed as %maximum in phsphorylation compared with those observed in LPS-stimulated control cells (n = 3). C: RAW264.7 cells, electroporated with control or SIRT1 siRNA, were stimulated with or without 100 ng/ml LPS for 1 h, and conditioned medium (CM) was harvested for ELISA analysis. Secreted TNFα was assayed for mouse TNFα using ELISA assay. Data are presented as percentages of secretion compared with LPS-stimulated control siRNA electroporated cells and represent means ± SE (n = 4). *P < 0.05, Etoh vs. RES or DMSO vs. SRT1720. †P < 0.05, control siRNA vs. SIRT1 siRNA.