Fig. 6.

SIRT1 activation leads to a reduction of CD11c expression in epididymal fat. Zucker fa/fa rats were placed on normal chow diet with vehicle (Zucker) or SRT2379 (Zucker + SRT), epididymal fat pad were isolated as described in Materials and Methods. A: representative histologic picture (hematoxylin and eosin staining) of the adipose tissue. Data are presented as percentages of mean area size of adipocyte compared with tissue from Zucker fa/fa rats and represent means ± SE. B–D: total RNA was purified, and then quantitative real-time-PCR was performed. After the mRNA expression differences were normalized to a standard housekeeping gene (GAPDH) mRNA level, data are presented as the relative expression. Error bars represent means ± SE from 4 independent experiments. Adipose tissue macrophage content was measured as described in Materials and Methods. Data are presented as percentages of macrophage per total nuclei and represent means ± SE. E: imunoblotting was performed with indicated antibodies. *P < 0.05, Zucker vs. Zucker + SRT. C: lean vs. Zucker or Zucker + SRT.