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. 2010 Jan 6;19(7):1153–1164. doi: 10.1093/hmg/ddp585

Figure 2.

Figure 2.

Minigene analysis of MBII-52 target genes. The exons harboring the MBII-52 complementary region were subcloned into the exon trap vector pSpliceExpress. The structure of the resulting constructs pSE-RALGPS1, pSE-CRHR1, pSE-DPM2, pSE-PBRM1 and pSE-TAF1 as well as the location of the primers used for RT–PCR analysis is indicated on the left. pEGFP: only an expression construct for GFP is transfected. All other lanes contain 1 µg of pSE-reporter. MBII-52: cotransfection with 2 and 4 µg of MBII-52 expression construct, MBII-85: cotransfection with 2 and 4 µg of an MBII-85 expression construct, MBII52cC: cotransfection with 4 µg of a C-box mutant of MBII-52: MBII52cD: cotransfection with 4 µg of a D-box mutant of MBII-52. The structure of the products is shown schematically on the right, using the same shading scheme as in Figure 1. The usage of alternative exons indicated with a triangle was statistically evaluated. The comparison between MBII-52 and MBII-85 transfected cells showed statistically significant differences, the P-values of the Student's t-test were: DPM2: 0.001, TAF1: 0.023; RALGPS: 0.021; PBRM1: 0.076 and CRHR1: 0.002; (n = 4).