Figure 5.

Analysis of proteins associated with MBII-52. (A) Experimental strategy: a biotinylated oligonucleotide is used to capture the snoRNP complex. The oligonucleotide is shown as a line, the complementarity is dashed, biotin is shown as a circle. The RNP complex (boxes) is isolated by streptavidin (half-circle)-capture from extracts expressing MBII-52 and washed in non-denaturing buffer. The extracts were prepared by transfecting MBII-52 expression constructs and performing Dignam mini-extracts. (B) Silver stain of a gel with affinity purified RNPs. MBII-52: the RNPs were isolated with a capture-oligonucleotide against MBII-52. MBII-85: the RNP was isolated with a capture oligonucleotide against MBII-85. Proteins were determined by mass spectrometry and are indicated. Sizes in kDa are shown in parentheses.