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. 2009 Dec 18;298(3):H736–H745. doi: 10.1152/ajpheart.00935.2009

Fig. 3.

Fig. 3.

Fractalkine (FKN) expression in HVSMCs under diabetic conditions. A: HVSMCs were incubated with Man (5.5. mM glucose + 19.5 mM mannitol), HG (25 mM glucose), or S100B (40 mg/l) for 8 h. Expression levels of FKN mRNA were quantified by real-time quantitative RT-PCR. Values are means ± SE (n = 5). *P < 0.001 vs. control. B: HVSMCs were incubated with Man, HG, or S100B for 48 h. Released FKN expression levels in the medium were measured using ELISA and then normalized to cell lysate protein concentration. Values are means ± SE (n = 5).*P < 0.001 vs. control. C: HVSMCs were incubated with Man, HG, or S100B for 48 h. Cell lysates (50 μg each) were used to determine cellular FKN expression levels in HVSMC by ELISA. Values are means ± SE (n = 5). *P < 0.01 vs. control.