Fig. 2.

HDL induces a time-dependent increase in the phosphorylation of MEK1/2 and ERK1/2 in adult mouse cardiomyocytes. Myocytes were exposed or not to 100 μg/ml HDL for different times (1–120 min; A and B). Western blot analyses of phosphorylated (p-)MEK 1/2, total (t-)MEK1/2, p-ERK1/2, and t-ERK1/2 representative of 3 independent experiments are shown. The band intensities of phosphoproteins were normalized against total MEK or ERK and expressed as fold increase over the zero time point. *P < 0.05 compared with all values without an asterisk.