Fig. 6.
Immunoprecipitation of IL-18 and the supernatant from resting PMNs immunoblotted for F-actin. No F-actin immunoreactivity (47 kDa) was present in the beads (B) or from an immunoprecipitation of p67phox, an antibody that is isotypically identical to the antibody employed for IL-18. F-actin immunoreactivity was precipitated by antibodies to IL-18 and was also present in the whole cell lysates (WC), which were used as a positive control MW, molecular weight. Figure is representative of 3 identical experiments with different donors.