Fig. 5.

Elevated expression levels of S and G₂/M phase associated cyclins A and B1 in hyperproliferating Cav-2 KO MLECs relative to their WT counterparts determined by immunoblotting. A: WT and Cav-2 MLECs were plated at 3 × 10⁵ per 150-mm dish, and 2 days later, cells were lysed and SDS-PAGE-resolved protein lysates were immunoblotted with the indicated cyclin-specific antibodies. B–E: mean densitometric ratios ± SD (n = 3) of cyclin proteins expression levels to protein loading control Hsp-90. Note a visible increase in the expression levels of cyclins A and B1 but not D1 and E in Cav-2 KO relative to WT MLECs.