Fig. 6.
Increased levels of phosphorylated/inactive form of the Rb protein with concurrent decrease in the expression levels of cyclin-dependent kinase (cdk) inhibitors p27Kip1 and p16INK4 in hyperproliferating Cav-2 KO relative to WT MLECs determined by immunoblotting. A: WT and Cav-2 MLECs were plated at 3 × 105 per 150-mm dish, and 2 days later, lysed and immunoblotted with the indicated antibodies. B: mean densitometric ratios ± SD (n = 3) of phosphorylated (inactive) Rb levels to protein loading control Hsp-90. C and D: mean densitometric ratios ± SD (n = 3) of indicated cdk inhibitors p16INK4 (p16) and p27Kip1 (p27) expression levels to protein loading control Hsp-90.