Fig. 4.

Phosphorylation of ANT at Y194 in HeLa cells. A: serum-starved HeLa cells stimulated with 0.1 mM pervanadate (Van) or 1 mM H₂O₂ for the indicated times before harvest. B: serum-starved HeLa cells were untreated (−) or pretreated with 10 μM Src-FK inhibitor PP2 (+) for 60 min and then stimulated with 0.1 mM pervanadate for 30 min or 1 mM H₂O₂ for 5 min before harvest. Cytosolic and mitochondrial fractions were separated and analyzed by Western blotting with anti-pY194, anti-phospho-Src family kinase (pSrc-FK^Y416), anti-ANT (Q-18), and anti-Src antibodies, as indicated.