Fig. 7.

Perturbation of the putative cytosolic salt bridge network by pY194. A: the putative cytosolic salt bridge network consisting of K95-D195, K198-D291, and K294-D92 ion pairs is marked by dashed green lines. Blue, positively charged residues; red, negatively charged residues. pY194 places a negative phosphate group (red circle) in the jaws of the K198-D291 salt bridge, decreasing the activation energy for the interconversion from the m- to the c-state. B: the phosphate group of pY194 interacts with K198, potentially annihilating its interaction with D291.