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. 2009 Dec 16;298(3):F745–F753. doi: 10.1152/ajprenal.00182.2009

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Fig. 1. — Protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) are present in rat brush-border membrane vesicles and expressed in OKP cells, a proximal tubule cell line. Thirty micrograms of rat cortical whole homogenate (WH), 30 μg of rat brush-border membrane vesicles (BBMV), 15 μg of OKP cells, or 15 μg of human embryonic kidney (HEK) cells were solubilized, separated by SDS-PAGE, and prepared for immunoblotting with anti-NHE3 antibody (A), anti-PP1α, anti-PP1β, or anti-PP1γ antibody (B), or anti-PP2A–C antibody (C).