Fig. 6.

CA inhibits PP1 and PP2A, whereas OkA inhibits only PP2A. OKP cells were treated with vehicle (C), CA for 30 min, or OkA for 30 or 60 min and then solubilized, subjected to SDS-PAGE, and immunoblotted with an antibody to either phospho-eukaryotic translation elongation factor 2 (p-eEF2; A), total eEF2 (B), phospho-Na⁺-K⁺-Cl⁻ cotransporter (p-NKCC1; D), or total NKCC1 (E). Densitometric analysis is shown for multiple similar experiments in which p-eEF2 (C) and p-NKCC1 signals (F) were quantitated and normalized to total eEF2 and total NKCC1, respectively. Data are means + SE; n = 4. P values were calculated using a paired t-test. *P < 0.05 compared with control.