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PAI-1 immunostaining at 12 wk after 5/6 Nx (control 5/6 Nx: A, B; ARB: C, D; AT2RA: E, F; combined: G, H). PAI-1 protein paralleled that of PAI-1 mRNA in glomeruli and the tubulointerstitial area. PAI-1 immunostaining was especially increased in sclerotic glomeruli and was markedly increased in control untreated 5/6 Nx and AT2RA-treated rats (A, B, E, F), with less staining in ARB and combined rats (C, D, G, H). A, C, E, G: ×400. B, D, F, H: ×200, anti-PAI-1 staining.
