Fig. 5.

AT₂R mRNA expression in the mouse metanephros on E11.5–E13.5 as determined by in situ hybridization (ISH). A–D: whole mount ISH. E and F: ISH on kidney sections. A: on E11.5, AT₂R mRNA is expressed predominantly in the UB stalk and mesenchyme (M) adjacent to the T-stage UB tip (blue staining). AT₂R mRNA is not present in the UB tips (outlined by dotted lines). C: on E12.5, AT₂R mRNA is expressed in the ureter, UB branches, and mesenchyme. E: on E13.5, AT₂R mRNA is present in the UB, mesenchyme, and maturing glomeruli (G). B, D, and F: control kidneys hybridized with the sense AT₂R probe show remarkably attenuated staining.