Fig. 6.
AT2R blockade downregulates Spry1, glial-derived neurotrophic factor (GDNF), c-Ret, and Wnt11 mRNA expression in E12.5 mouse metanephroi that were grown ex vivo for 24 h. After 24 h in culture, kidney explants were processed for whole mount in situ hybridization or total RNA was extracted and Spry1, GDNF, c-Ret, and Wnt11 mRNA levels were analyzed by real-time quantitative RT-PCR. A: representative images demonstrate that treatment with PD123319 decreases the expression of c-Ret and Wnt11 in the UB tips, of GDNF in the mesenchyme, and of Spry1 in the UB tips and mesenchyme. Note that GDNF staining in the UB is nonspecific. B: bar graph showing that treatment with PD123319 decreases Spry1, GDNF, c-Ret, and Wnt11 mRNA levels as determined by RT-PCR.