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. 2009 Dec 23;298(3):F807–F817. doi: 10.1152/ajprenal.00147.2009

Fig. 7.

Fig. 7.

Effect of AT2R antagonist PD123319 on UB cell apoptosis and proliferation. A–D: apoptotic cells were identified by terminal uridine triphosphate (UTP) nick-end labeling (TUNEL; brown staining). A: media. B and C: PD123319 (10−6 M). D: kidney treated with TACS-nuclease to generate DNA breaks in every cell (positive control). E: bar graph shows the effect of media or PD123319 on the number of TUNEL-positive cells in the UB tip and stalk cells. F–I: proliferating cells are identified by anti-phospho-histone H3 (pH3) antibody (red staining). UB epithelia are visualized with anti-cytokeratin antibody (green). F and G: media. H and I: PD123319 (10−6 M). PD123319-treated metanephroi have less pH3 staining in UB (arrows) compared with control (media; marked by rectangles in G and I). J: bar graph shows the effect of media or PD123319 on cell proliferation index in the UB tip and stalk cells.