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Western blot analysis of NKCC2 in outer medulla (OM). Provided are 3 representative samples of IM tip lysates. C, 7D, 14D, 7R, and 14R represent the same experimental groups defined in Fig. 1. The bar graph represents densitometry values calculated based on tubulin-loading controls that have been normalized to percent of control. Values are SE from each experimental group where n = 12. *P < 0.05 vs. controls. §P < 0.05 vs. 14D.
