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Paired box gene 8 (PAX8) staining in transforming growth factor (TGF)-β1 transgenic (Tg) mice. PAX8 staining was performed to evaluate the change in the number of parietal epithelial cells (PECs) in wild-type (WT; A and C) and TGF-β1 Tg mice (B and D). C and D: the primary antibody was omitted as a control. E: statistical analysis showed a significant increase in the number of PECs in TGF-β1 Tg mice at both 3 and 5 wk of age.
