Table 1.
Podocyte marker expression
| CD2AP |
Nestin |
Synaptopodin |
Nephrin |
Podocalyxin |
WT1 |
Podocin |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell Line | Subject | mRNA | Protein | mRNA | Protein | mRNA | Protein | mRNA | Protein | mRNA | Protein | mRNA | Protein | mRNA | Protein |
| FSGS patient A | 1 | + | + | + | + | + | + | + | ND | + | + | − | − | − | ND |
| 2 | + | ND | + | ND | + | ND | + | ND | + | ND | − | − | − | ND | |
| FSGS patient B | 1 | + | + | + | + | + | + | + | ND | + | + | + | + | − | ND |
| 2 | + | ND | + | ND | + | ND | + | ND | + | ND | + | + | − | ND | |
| Healthy volunteer A | 1 | + | + | + | + | + | + | − | ND | − | ND | + | − | − | ND |
| 2 | + | ND | + | ND | + | ND | + | ND | − | ND | + | ND | − | ND | |
| Healthy volunteer B | 1 | + | + | + | + | + | + | + | ND | + | ND | + | — | − | ND |
| 2 | + | ND | + | ND | + | ND | + | ND | + | ND | + | ND | − | ND | |
Two cell lines were obtained from subjects with focal segmental glomerulosclerosis (FSGS) and healthy volunteers. WT1, Wilms' tumor 1. In cells cultured under growth-restricted conditions, mRNA expression was determined by PCR, and protein expression was determined by Western blotting and/or immunostaining. Results are presented as present (+), absent (−), or not determined (ND).