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Time course of renal cortical TNF-α mRNA levels and RNA polymerase II (Pol II) binding to the TNF-α gene. After glycerol injection, TNF-α mRNA was markedly elevated and continued to increase over the 7 days of observation. A corresponding progressive increase in Pol II binding to exon 1 of the TNF-α gene was also observed. Open bars, controls; solid bars, glycerol-treated mice.
