Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Dec 23;298(3):F827–F837. doi: 10.1152/ajprenal.00683.2009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010 the American Physiological Society

PMC Copyright notice

Fig. 11. — Effects of Fe on mRNA stability in HK-2 cells incubated overnight under control conditions or with FeG or FeS. Half of the cells in each group were then treated for 6 h with actinomycin D (AD) to inhibit new mRNA synthesis. Thus, changing mRNA levels in the presence of actinomycin D reflects mRNA degradation rates. Percentage of mRNA remaining in actinomycin D-treated cells was then calculated as follows: (mRNA in actinomycin-treated cells ÷ amount of mRNA in control incubated cells) × 100%. Extent of mRNA reductions in control cells (C) greatly varied (TNF-α manifested the greatest decline, followed in order by HO-1, MCP-1, and TGF-β1 mRNAs). In no instance, did Fe pretreatment affect these results (indicating no Fe-mediated mRNA stabilization).