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Time course of histone 2 variant H2A.Z and histone 3 lysine 4 (H3K4) methylation at exon 1 of the TNF-α gene. A progressive increase in levels of H2A.Z was observed 1, 2, and 7 days after glycerol injection. H3K4 was probed with an antibody that recognizes mono-, di-, and trimethylation, and a progressive increase in the sum of these 3 moieties was observed over the 7 days of observation. Open bars, controls; solid bars, glycerol-treated mice.
