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H3K4m3 7 days after glycerol treatment. To assess whether H3K4m3, in particular, contributed to the overall increase in H3K4 methylation observed with the anti-H3K4m1 + m2 + m3 antibody, samples were probed with an antibody specific for H3K4m3 (a gene-activating histone mark). Assessments were restricted to kidney samples obtained on day 7. H3K4m3 was increased at each of the 4 genes. Open bars, controls; solid bars, glycerol-treated mice.
