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Effect of sex hormones and/or NE treatment on NRVM sarcomeric organization. Cells were treated with sex hormones alone, NE alone, or NE with sex hormones for 48 h (protocol 1). Confocal micrographs of myocytes after incubation with E2, DHT, and/or NE for 48 h. Actin is visualized with FITC-conjugated phalloidin stain (green), and the nucleus is visualized by 4′,6′-diamidino-2-phenylindole staining (blue).
