Fig. 4.

Effect of long-term (48 h) sex hormone exposure on rapid (2 min) NE-elicited alterations in ERK and focal adhesion kinase (FAK) phosphorylation. NRVMs were incubated with either E2 or DHT for 48 h and then stimulated with NE (or diluent, control) for 2 min (protocol 2). A: schematic illustration of protocol 2. B: typical Western blots. C and D: mean (±SE) phosphorylated total ERK (pERK; C) and phosphorylated FAK (pFAK; D) normalized to GADPH and expressed as a percentage of control from 3 separate experiments. *P < 0.05, significantly different from control. #P < 0.05, significantly different from NE alone. @P < 0.05, significant difference between sex hormone treatments.